Genetics and biotechnology of the H(2)-uptake [NiFe] hydrogenase from Rhizobium leguminosarum bv. viciae, a legume endosymbiotic bacterium.
نویسندگان
چکیده
A limited number of strains belonging to several genera of Rhizobiaceae are capable of expressing a hydrogenase system that allows partial or full recycling of hydrogen evolved by nitrogenase, thus increasing the energy efficiency of the nitrogen fixation process. This review is focused on the genetics and biotechnology of the hydrogenase system from Rhizobium leguminosarum bv. viciae, a frequent inhabitant of European soils capable of establishing symbiotic association with peas, lentils, vetches and other legumes.
منابع مشابه
Functional and expression analysis of the metal-inducible dmeRF system from Rhizobium leguminosarum bv. viciae.
A gene encoding a homolog to the cation diffusion facilitator protein DmeF from Cupriavidus metallidurans has been identified in the genome of Rhizobium leguminosarum UPM791. The R. leguminosarum dmeF gene is located downstream of an open reading frame (designated dmeR) encoding a protein homologous to the nickel- and cobalt-responsive transcriptional regulator RcnR from Escherichia coli. Analy...
متن کاملThe Rhizobium Ieguminosarum biovar viciae nod0 gene can enable a nod€ mutant of Rhizobium leguminosarum biovar trifolii to nodulate vetch
Sciences, University of East Anglia, Norwich NR4 7TJ, UK 2 School of Biological Analysis of the nodulation characteristics of transposon-induced mutants of Rhizobium leguminosarum bv. viciae revealed that nodO and the closely-linked rhi genes contribute t o nodulation of peas (Pisum sativum) and the vetch Vicia hirsuta. Although mutation of nodO alone had no significant effect on nodulation of ...
متن کاملHost-dependent expression of Rhizobium leguminosarum bv. viciae hydrogenase is controlled at transcriptional and post-transcriptional levels in legume nodules.
The legume host affects the expression of Rhizobium leguminosarum hydrogenase activity in root nodules. High levels of symbiotic hydrogenase activity were detected in R. leguminosarum bacteroids from different hosts, with the exception of lentil (Lens culinaris). Transcription analysis showed that the NifA-regulated R. leguminosarum hydrogenase structural gene promoter (P(1)) is poorly induced ...
متن کاملGenomic Diversity in the Endosymbiotic Bacterium Rhizobium leguminosarum
Rhizobium leguminosarum bv. viciae is a soil α-proteobacterium that establishes a diazotrophic symbiosis with different legumes of the Fabeae tribe. The number of genome sequences from rhizobial strains available in public databases is constantly increasing, although complete, fully annotated genome structures from rhizobial genomes are scarce. In this work, we report and analyse the complete g...
متن کاملRhizobium leguminosarum hupE encodes a nickel transporter required for hydrogenase activity.
Synthesis of the hydrogen uptake (Hup) system in Rhizobium leguminosarum bv. viciae requires the function of an 18-gene cluster (hupSLCDEFGHIJK-hypABFCDEX). Among them, the hupE gene encodes a protein showing six transmembrane domains for which a potential role as a nickel permease has been proposed. In this paper, we further characterize the nickel transport capacity of HupE and that of the tr...
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 33 Pt 1 شماره
صفحات -
تاریخ انتشار 2005